Fig. 6

miR-148a-3p participates in the progression of glioma cell tumorigenesis and angiogenesis in vivo. The nude mice were injected with U251 cells to form xenografts and then injected with miR-148a-3p agomir, miR-148a-3p antagomir, or miR-148a-3p antagmir + U251-exo. a Representative images showing tumors formed in nude mice. b The volume of tumors in nude mice recorded every 7 days. c Tumor weight of nude mice. d IHC to quantify tumor MVD. e VEGF and VEGFR2 expression changes in nude mice detected by ELISA. f miR-148a-3p and ERRFI1 expressions were determined by RT-qPCR in tumor tissues of nude mice (U6 and GAPDH were used as internal controls, respectively). g Western blot analysis to detect ERRFI1, p-EGFR/EGFR, p-ERK1/2/ERK1/2 protein expressions in tumor tissues of nude mice. h Expression of miR-148a-3p was determined by RT-qPCR in serum exosomes in nude mice (cel-miR-39 was used as an internal reference). U251 mentioned in this figure refers to U251-MG. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. Data are shown as mean ± SEM (n = 10). Repeated measures ANOVA was used for data analysis among multiple groups at different time points. One-way ANOVA was used for multi-group comparison with Tukey's test