Fig. 2

Upregulated miR-365 reverses the epithelialization of GES-1 cells induced by TNF-α. a miR-365 expression in TNF-α-induced GES-1 cells detected by RT-qPCR upon treatment with miR-365-mimic or NC-mimic; b Viability of TNF-α-induced GES-1 cells upon treatment with miR-365-mimic or NC-mimic measured by CCK-8 assay; c Apoptosis of TNF-α-induced GES-1 cells upon treatment with miR-365-mimic or NC-mimic measured by flow cytometry; d IL-1 and IL-6 contents of TNF-α-induced GES-1 cells upon treatment with miR-365-mimic or NC-mimic measured by ELISA; e Western blot analysis of E-cadherin, N-cadherin, Bcl-2 and Bax proteins in TNF-α-induced cells upon treatment with miR-365-mimic or NC-mimic; * p < 0.05, vs. NC group. Measurement data (mean ± standard deviation) between two groups were compared with unpaired t test, while data between multiple groups were compared with one-way ANOVA; data comparison between groups at different time points was compared with two-way ANOVA. Cell experiments were repeated three times