Fig. 4

MiR-5590-3p downregulates the ERK/MAPK signalling pathway by targeting OTX1. a The predicted miR-5590-3p binding sites in OTX1. b The luciferase activity of OTX1-WT and OTX1-MUT reporter constructs in HEK293T cells transfected with the miR-5590-3p mimics and inhibitor. c The mRNA levels of OTX1 in TNBC cells transfected with the miR-5590-3p mimics and inhibitor, as detected by RT-qPCR. d, e The protein levels of OTX1 in TNBC cells transfected with the miR-5590-3p mimics and inhibitor, as detected by western blot analysis. f, g The protein level of ERK/MAPK signalling pathway after transfection, as detected by western blot analysis. *P < 0.05, **P < 0.01, ***P < 0.001