Fig. 4

Co-culture with adipose extract enhanced energy and lipid metabolism in colon cancer cells. The HCT-8 and HCT-116 cells were cultured in medium with different treatments (complete medium, medium containing adipose extract (80 mg/mL) and medium containing adipose extract (80 mg/mL) plus 20 μM/L BMS309403). a The lysates of cells were collected for intracellular ATP detection. b–d The culture medium of cells was collected for extracellular LDH, glycerol and NEFA detection. All the samples were prepared in triplicate, and all experiments were repeated for at least three times. *P < 0.05