Fig. 1

Characteristics of hsa_circ_0026416. a Heat map showing differentially expressed circRNAs in the GSE77661 colorectal tissue related dataset. b The illustration shows that the cyclization of KRT6C exons 3, 4, 5, 6, 7 and 8 form hsa_circ_0026416. c Sanger sequencing validates hsa_circ_0026416 splicing junction. d Expression levels of hsa_circ_0026416 in 169 cases of CRC tissues and their ANM using qRT-PCR. e Kaplan–Meier overall survival curves were created for CRC patients (n = 169) and were split into low and high hsa_circ_0026416 expression groups. f Hsa_circ_0026416 expression levels were detected by qRT-PCR in a normal intestinal epithelial cell line (HCO) and CRC cell lines DLD-1, HCT-116, HCT-8 and SW480. g, h qRT-PCR analysis of hsa_circ_0026416, GAPDH, and U6 in the nucleus and cytoplasm in HCT-8 and SW480 cells. Data are shown as means ± SD of three independent experiments. ns = not significant, **P < 0.01, ***P < 0.001