Fig. 5

Hsa_circ_0026416 promotes CRC progression by a ceRNA pattern in vitro. a–d qRT-PCR showing that NFIB and miR-346 are upregulated in HCT-8 and SW480 cells after pcDNA3.1-NFIB, and miR-346 mimics transfection also downregulated expression in HCT-8 and SW480 cells after si-NFIB and miR-346 inhibitor transfection. e–h Transwell and CCK-8 assays indicating that pcDNA3.1-NFIB rescues the inhibitory function of miR-346 mimics on migratory, invasive and proliferative activity in HCT-8 and SW480 cells. i–l Transwell and CCK-8 assays indicating that si-NFIB rescues the enhanced function of miR-346 inhibitor on migratory, invasive and proliferative activity of HCT-8 and SW480 cells. Data are presented as means ± SD of three independent experiments. *P < 0.05, **P < 0.01, *** or ****P < 0.001