Fig. 2

PD enhanced CDDP-induced apoptosis and DNA damage in MDA-MB-231 cells in the PD-CDDP group. a Flow cytometry showed that PD enhanced the CDDP-induced apoptosis of MDA-MB-231 cells in the PD-CDDP group, and the average percentage of cells in apoptosis was quantified. b–d Immunofluorescence analysis was used to evaluate the expression of γH2AX (green) in MDA-MB-231 cells treated with PD, CDDP, PD + CDDP, PD-CDDP and CDDP-PD. DAPI was used to detect nuclei. Images were captured at × 400 with an Olympus microscope