Skip to main content
Fig. 6 | Cancer Cell International

Fig. 6

From: LncRNA NEAT1 promotes proliferation, migration, invasion and epithelial-mesenchymal transition process in TGF-β2-stimulated lens epithelial cells through regulating the miR-486-5p/SMAD4 axis

Fig. 6

Effects of miR-486-5p overexpression and SMAD4 overexpression on the progression of TGF-β2-stimulated SRA01/04 cells. a SRA01/04 cells were transfected with SMAD4 overexpression vector or pcDNA, and then treated with 10 ng/mL TGF-β2. WB analysis showed that the protein level of SMAD4 was promoted by SMAD4 overexpression vector (P < 0.0001) in TGF-β2-treated SRA01/04 cells. b–f SRA01/04 cells were co-transfected with miR-486-5p mimic and SMAD4 overexpression vector or pcDNA, and then treated with 10 ng/mL TGF-β2. MTT assay (b) and transwell assay (c, d) suggested that overexpressed SMAD4 reversed the inhibition effect of miR-486-5p on the proliferation (P < 0.0001), migration (P < 0.01, P < 0.001) and invasion (P < 0.0001) (100 μM) of TGF-β2-stimulated SRA01/04 cells. e WB analysis revealed that the regulation of miR-486-5p on the protein levels of E-cadherin, Vimentin and α-SMA could be reversed by SMAD4 overexpression (P < 0.0001) in TGF-β2-stimulated SRA01/04 cells. f WB analysis showed that miR-486-5p inhibitor reversed the decreasing effect of NEAT1 silencing on SMAD4 protein expression (P < 0.0001). **P < 0.01, ***P < 0.001, ****P < 0.0001

Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us