Fig. 3

LINC00152 promotes EMT and EC cell resistance to L-OHP by interacting with EZH2. a localization of LINC00152 in cytoplasm and nucleus in Kyse-150 and TE-1 cells determined by FISH assay (400 ×). b–c the interaction between LINC00152 and PRC2 in Kyse-150 and TE-1 cells determined by RIP assay. d binding of LINC00152 and PRC2 detected by RNA pull-down assay. e the mRNA expression of EZH2 in EC issues detected by RT-qPCR assay. f the mRNA expression of EZH2 in in Kyse-150 and TE-1 cells detected by RT-qPCR assay. g–h the protein expression of EZH2 in Kyse-150 and TE-1 cells measured by western blot analysis. i, J cell survival rate after 72 h of treatment with different concentrations of L-OHP (0, 0.5, 1.0, 2.5, 5.0, 10.0 µM) in Kyse-150 and TE-1 cells detected by CCK-8 assay. k, l the protein expression of E-cadherin, vimentin, cleaved PARP/PARP and cleaved Caspase 3/Caspase 3 after 72 h of treatment with 10.0 µM of L-OHP, determined by Western blot analysis. In panel b–c, * p < 0.05 vs. IgG. In panel e–g * p < 0.05 vs. normal adjacent tissues or Het-1A cells. * p < 0.05 vs. cells transfected with oe-LINC00152, # p < 0.05 vs. cells transfected with oe-LINC00152 and si-EZH2. Paired data in compliance with normal distribution and homogeneity between cancer tissues and adjacent tissues were compared using paired t-test. Comparisons among multiple groups were conducted by ANOVA with Tukey’s post hoc test. Data at different time points and different concentrations were compared by repeated measures ANOVA. Data are presented as mean ± standard deviation of three technical replicates