Fig. 4

SNHG17 upregulates CXCL12 through absorbing miR-23a-3p. a The PITA, miRmap, microT, miRanda, PicTar and TargetScan databases were used to predict underlying 18 mRNAs genes. b RT-qPCR tested the expression of 18 mRNAs in CRA cell lines compared to normal cell line FHC. c The expression of seven candidate mRNAs was assessed by RT-qPCR in the case of miR-23a-3p mimics. d, e RIP assay and RNA pull down assay confirmed the association among SNHG17, miR-23a-3p, CXCL12. f, g. Luciferase reporter assays were used to confirm the interaction of miR-23a-3p with SNHG17 or CXCL12. h The luciferase activity of CXCL12-WT or CXCL12-Mut was measured in cells co-transfected with miR-23a-3p mimics and pcDNA3.1/SNHG17 by luciferase reporter assay. i Tube formation assay verified the effect of silenced SNHG17 on the tube formation of CRA cells. Scale bar, 100 μm. **P < 0.01