Fig. 5

MiR-139 inhibits proliferation and migration of OC cells and promotes apoptosis by repressing LPA1. a The mRNA expression pattern of LPA1 in the OC tissues and adjacent normal tissues as determined by RT-qPCR (n = 69). b The protein expression pattern of LPA1 in OC tissues and adjacent normal tissues as measured by western blot analysis (n = 69). c The correlation between miR-139 expression and LPA1 expression pattern via Pearson correlation analysis. d The binding relationship between LPA1 and miR-139 verified by dual-luciferase reporter gene assay. e–i, SKOV3 cells were treated with miR-139 mimic alone or in the presence of oe-LPA1. e The protein expression pattern of CyclinD1, CDK2, MMP-2, MMP-9, Cleaved Caspase-3, Bax, and Bcl-2 in cells as assessed by western blot analysis. f Cell proliferation ability detected by EdU assay (×200). g Cell migration ability determined by Transwell assay (×100). H, cell invasion ability monitored using Transwell assay (×100). I, cell apoptosis evaluated using Flow cytometry. *p < 0.05 vs. control or mimic NC, ^#p < 0.05 vs. miR-139 mimic group. The measurement data were expressed as mean ± standard deviation. The paired-designed data was compared using paired t-test. The one-way analysis of variance was adopted for comparison among multiple groups, followed by Tukey’s post hoc test. The experiment was conducted 3 times independently