Fig. 6

Overexpression of miR-139 blocks the TGF-β signaling pathway activation by down-regulating LPA1. SKOV3 cells were treated with miR-139 mimic alone or in the presence of oe-LPA1. a the miR-139 expression pattern and the mRNA expression pattern of LPA1 of cells as determined by RT-qPCR; b the protein expression pattern of TGF-β1, Smad3, p-Smad3, Smad2, p-Smad2, ERK, p-ERK, JNK, p-JNK, p38, p-p38 in cells as measured by Western blot analysis; *p < 0.05 vs. mimic NC group, ^#p < 0.05 vs. miR-139 mimic group. The measurement data were expressed as mean ± standard deviation. The one-way analysis of variance was adopted for comparison among multiple groups, followed by Tukey’s post hoc test. The experiment was conducted 3 times independently