Fig. 8

Silencing of EZH2 inhibits tumor growth in vivo by elevating miR-139 expression. The tumor xenograft model was established by injecting the nude mice with the cells stably transfected with shRNA-NC and shRNA-EZH2. a The mRNA expression pattern of EZH2 and miR-139 in the tumor tissues of nude mice determined by RT-qPCR. b The protein expression patterns of EZH2, LPA1, TGF-β1, p-of Smad3 and p-Smad2 in the tumor tissues of nude mice as measured by western blot analysis. c The representative images of tumor in nude mice. d The tumor volume in nude mice at the 7th, 14th, 21st, and 28th d after injection. e Tumor weight of nude mice. *p < 0.05 vs. mimic-NC group, ^#p < 0.05 vs. miR-139 mimic group. The measurement data were expressed as mean ± standard deviation. The statistical significance of the data (n = 16) between two groups was calculated using the unpaired t-test