Fig. 6
From: LncRNA LEF1-AS1 promotes metastasis of prostatic carcinoma via the Wnt/β-catenin pathway
LEF1-AS1 promotes AIPC aggressiveness by targeting FZD2 and CD44. a–c Colony formation (a), transwell (b) and tube formation assays (c) were performed to assess the function of LEF1-AS1 in AIPC cells after FZD2 or CD44 knockdown. Cotransfection with LEF1-AS1 and siFZD2/siCD44 partly reversed the oncogenic effects induced by LEF1-AS1 overexpression. ***P < 0.001. d, e The expression of β-catenin and downstream targets (MMP-7 and c-myc) was also decreased after siFZD2/siCD44 transfection in LEF1-AS1-overexpressing cells compared to that observed in the control group. f The effect of LEF1-AS1 on the expression of β-catenin was determined by western blot. g Mechanism for the regulatory function of LEF1-AS1 in AIPC progression