Fig.1

Timeline of in vitro experiments. a Treatment response of HER2 + breast cancer cells in response to incremental doses of trastuzumab (0–100 µg/mL) for 24 h. b Quantifying immune influence of CD4 + T-cells on HER2 + breast cancer in response to single agent trastuzumab. c Evaluating timing of T-cell co-culture on HER2 + breast cancer’s response to trastuzumab. CD4 + T-cells were introduced into cell culture at t = 0 h during initial plating of cells or t = 24 h during trastuzumab treatment and longitudinal changes in cell viability was assessed. d Evaluating TNF-α effect on trastuzumab induced HER2 receptor blockade