Fig.4

Representative images of T-cell and cancer cell co-culturing with fluorescence segmentation and HER2 quantification. GFP and RFP segmented images of breast cancer cells treated with 25 µg/mL trastuzumab when cultured without (a) and with (b) CD4 + T-cells. c Correlation of HER2 expression and significance between changes in viability of trastuzumab treated cancer cells and trastuzumab treated co-cultured cells. The fold change in cell viability has a negative correlation to HER2 expression (r² = 0.49). d Normalized HER2 expression was identified with western blots. High HER2 overexpression was observed in BT474 and SKBR3 cell lines. Moderate HER2 overexpression was observed in MDA-MB-453 cell lines. Low HER2 overexpression was observed in MDA-MB-231 cell lines