Fig. 1

FOXM1 regulates apoptosis and cell cycle in docetaxel-treated PCa cells. a VCaP and DU145 cells were transfected with a FOXM1 plasmid, using lipofectamine 2000; western blotting (WB) confirmed overexpression of FOXM1 protein. b Cells were transfected with FOXM1 plasmid and treated with docetaxel (DOC, 25 nM). Apoptosis was detected using flow cytometry (FCM). c VCaP-DR and DU145-DR cells were transiently transfected with siRNA oligonucleotides targeting FOXM1 (siFOXM1) using lipofectamine 2000 and verified using WB. d FCM assessed cell apoptosis after FOXM1 knockdown alone or in combination with DOC (25 nM and 50 nM). e FOXM1 was depleted in VCaP-DR cells or in combination with DOC (25 nM). FCM was used to assess the cell cycle. f VCaP cells was transfected with pc-FOXM1 or combined with DOC (25 nM). Cell cycle distribution was determined by FCM. Data represent the mean ± SD of three independent experiments. ^*p < 0.05, ^**p < 0.01