Fig. 3

FOXM1 inhibition contributes to docetaxel sensitiveness in vitro and in vivo. a VCaP-DR and DU145-DR cells was treated with TST (3 μM). WB was used to analyze FOXM1 protein levels. b MTT was used to assess cell proliferation after administration of different thiostrepton (TST) doses (1 μM, 2 μM, 3 μM, 4 μM, 5 μM) for 72 h in VCaP-DR and DU145-DR cells. c Resistant cells were treated with different doses of thiostrepton (TST), docetaxel (DOC), or their combination. Cell viability was detected using MTT. D-F: Tumor xenografts were established in immunodeficient nude mice injected with DU145-DR cells. Nude mice with tumor xenografts were treated with or without DOC (10 mg/kg) by i.p. injection twice a week or TST (30 mg/kg) by i.p. injection every-other-day. The xenograft tumor size was measured every four days. d The tumor masses were harvested from immunodeficient nude mice at the end of the experiments. e The weight of tumors was measured and averaged for comparison between groups. f Data represents the mean ± SD (n ≥ 3). ^*p < 0.05, ^**p < 0.01