Fig. 9

Transwell invasion test on glioblastoma (NG97) cells after 12 and 48 h of treatments. Cells received a ROCK inhibitor prior and during the treatments. a and e—Cells maintained in medium (Control); B and f—Cells treated with PnV at 14 μg/ml. c and g Cells incubated with SF1 (1.0 μg/ml); d and h—Cells treated with SF11 (1.0 μg/ml). Note that, even when ROCK was inhibited, cells treated with PnV, SF1 and SF11 showed a significantly reduction in the number of migrating cells (both 12 and 48 h) compared to Control. Graphs i and j present the statistical comparison, where *P < 0.05 and **P < 0.01, compared to control. Results of three independent experiments. Bars = 100 µm