Fig. 7

Effects of miR-584-5p and circSMYD4 on tumor formation and apoptosis-related genes in vivo. a–c Nude tumor formation experiments were conducted to examine the effects of miR-584-5p and circSMYD4 on tumor formation in vivo. d In SNU-387 or SK-Hep1 cells, the mRNA expressions of Bcl-2 and Bax in the NC + MC, circSMYD4 + MC, NC + M, and circSMYD4 + M groups were analyzed by RT-qPCR. e, f Western blot was performed to detect the expressions of cleaved Caspase-3, Bcl-2 and Bax proteins in SNU-387 or SK-Hep1 cells in each group. Both biological and technical replicates were examined three times. GAPDH was used as a control. Choose the three most representative data from the 8. The experiment was repeated for 3 times. ^**p < 0.01, ^***p < 0.001 vs. NC + MC; ^{^^}p < 0.01, ^{^^^}p < 0.001 vs. circSMYD4 + MC; ^##p < 0.01, ^###p < 0.001 vs. NC + M. RT-qPCR: reverse transcription real time quantitative polymerase chain reaction