Table 1 The sequences of primers used in RT-qPCR
From: ETS1-activated SNHG10 exerts oncogenic functions in glioma via targeting miR-532-3p/FBXL19 axis
Primers | Sequences (5′–3′) |
|---|---|
SNHG10-F (forward) | CAAGCCTCATCAGGCCCATT |
SNHG10-R (Reverse) | AGTCCACTGGTCCTGCTCTT |
OCT4-F | CCTTCGCAAGCCCTCATTTC |
OCT4-R | TAGCCAGGTCCGAGGATCAA |
Nanog-F | CCCCTAATTTGTTGGTTGTGCT |
Nanog-R | GCTAATTTCCTTCTCCACCCCA |
SOX2-F | TGGACAGTTACGCGCACAT |
SOX2-R | CTCGGACTTGACCACCGAAC |
GAPDH-F | GGAGCGAGATCCCTCCAAAAT |
GAPDH-R | GGCTGTTGTCATACTTCTCATGG |
U6-F | CTCGCTTCGGCAGCACA |
U6-R | AACGCTTCACGAATTTGCGT |
FBXL19-F | AACTCATCTCCAGCCCTCCTT |
FBXL19-R | CCATCCATGGGGCTAAGGG |
NPTN-F | GGAAGACCCTGGCGAGTATG |
NPTN-R | TCCAGCTGGTTCATCATCGT |
SRRM2-F | ATGAGACACCGCTCCTCCA |
SRRM2-R | GCTGCCAAGGTTCAAAGGAG |
ETS1-F | TGTAGCGATGTAAGTGTCGATG |
ETS1-R | GATGTGCCAGCATCAGCTAC |
miR-2278-F | ACGAGGAGAGCAGTGTGTGTT |
miR-532-3p-F | ATAATCCTCCCACACCCAAGG |
miRNA-R (universal) | CTCAACTGGTGTCGTGGA |