Table 2 The sequences of plasmids applied for cell transfection
From: ETS1-activated SNHG10 exerts oncogenic functions in glioma via targeting miR-532-3p/FBXL19 axis
Plasmids | Sequences (5′–3′) |
|---|---|
sh-NC (for SNHG10) | CCGGACATGACTGTATCTGTCTAGTCTCGAGACTAGACAGATACAGTCATGTTTTTTG |
sh-SNHG10#1 | CCGGGCAACCGCTTTGTTAGTTAATCTCGAGATTAACTAACAAAGCGGTTGCTTTTTG |
sh-SNHG10#2 | CCGGGCGCGCGATTATTTCTCTAGACTCGAGTCTAGAGAAATAATCGCGCGCTTTTTG |
sh-NC (for FBXL19) | CCGGCGAGACATAGGATCGGCAGATCTCGAGATCTGCCGATCCTATGTCTCGTTTTTG |
sh-FBXL19#1 | CCGGGCCGAGACATGAAGAAGTTCGCTCGAGCGAACTTCTTCATGTCTCGGCTTTTTG |
sh-FBXL19#2 | CCGGGCTGGATCGAGGATGTTAAAGCTCGAGCTTTAACATCCTCGATCCAGCTTTTTG |
sh-NC (for ETS1) | CCGGATCAGAGTACTCCTACGCTGACTCGAGTCAGCGTAGGAGTACTCTGATTTTTTG |
sh-ETS1#1 | CCGGGCAACTCAGGAAGTTCCTACTCTCGAGAGTAGGAACTTCCTGAGTTGCTTTTTG |
sh-ETS1#2 | CCGGGGAAGTTCCTACTGGTCTTGACTCGAGTCAAGACCAGTAGGAACTTCCTTTTTG |
NC mimics/inhibitor | CCAAGCCACCCUACCGCUUGCA |
miR-532-3p mimics | CCUCCCACACCCAAGGCUUGCA |
miR-532-3p inhibitor | UGCAAGCCUUGGGUGUGGGAGG |
pcDNA3.1/SNHG10 | AGCCTCATCCTACTGCCTTACTATTGGTCGTCGGCAACCGCTTTGTTAGTTAATTGGAGAGACTGGATGTCCATCAGGGTCACGCTTGCAGGGCAACGAGAGGCAAAACAAGAGGGAAGACGACTTTCCTCCTGTGAGCCAATGAGGCCAGCTGGACTACGCCGAGACAACTGGGAGAGGCGCGGGACTCGCCCGTTCCGCGGAACGCCGGGAAGGGGTCACCTCCTGATGAAGTTTCCGGTTCCGGTGTCAGCGGCGGTTGAATTGCCATGGCAATGCGGTGGGCGCGCGCTTGTCGTGTTGGTCTCTTGGGAGGTAGCGGGGCTAGGCCGGGCGGGTATCCGCCTCTCCCAGCTTAGGTGAGCGTCCCCGGGCGCCTCCGGAGCGCCGCGGCCGCATGCAGTTCGTCGTGGCGGGGAGCCGGAGCCTGACCGGGGTTCCAGCGCTCGGGCCGTAGCCTTGGCTCCTGGACTTTCCCTGGCTCCGCCGCCACGTGGGAGCTGAGGCTCTGGGGCTTCCGCCTCCGGCGCGCGATTATTTCTCTAGAACAGTTTTCATTTTTAAAATTTGTAAAGCGCTTTTGCCTGTGTGATTTCCTCTGGGTTTTTTTTTTTTTTCTTCCTTTTTGTAGAGACGGAATTGGCGGCGGGGGCGGGGGGTCGATGTCTCACTTTTTTGCCCAGGCTGGTCTCGAACTCCTGGCTTCAAGGGATCCTCCTGCCTCGGCCTCTTAAAGTGCTGGGATTACAGGCGTGAGCCACCGCCCCCGGCCGCCTCTGAGTTTCCAGCCTCGTTGGCCCTCCAGCCTTTTAACCTGTTGGGCCTAGGATCAGGAAAGGTTTGTTGAATGGGGAACTAAGAAGTGAATTCGTTCGTTCGACAAACGTTTCCTGAGCAGCCGCTGGGTGCTAGGCGCAGTGCCAGCGCGGAATGTCCAGGGAGACCTGGTGCCCAAAGCTTGGACCCATCGTGAGAAATGAGAAGCAGATACAAAGCAGTGTGGGAGTGCAGAGGAGACAAAGCAAGCCTCATCAGGCCCATTGCTTGCTCTGCTCTCCCTTGTACTTACCAGTGCTTGACAATATACAGTTATTTACTAGCTTGGTTATTGACTTCCTACCCAGCACTCAGTTTTATTCACTGCTGTATCCTCAGTGCCTAGGACGATGCTTGGAACGTGGTAAGTGCTCCTATTGGCGGGAAGAATAAATCCGGAAGAGCAGGACCAGTGGACTTGCTACATAATCTGTAGTCTTGGAGCCGCACAGGGTTGGTGGTACCCTCGAGCACACCAGACTTGCAGAAAAAGCATACTCCAGAGGAAGCTGAGGCATGCCTGCTCGAGAGCCAGCTGTTCCATGTGCAATTTTCCTCTGATAGTTTCTGGTCACTGTTGCCACGGTGATAATGACTGGGCTATGTCATTATCTATCCGCCAACAGTAAGAGAAGCTTTGCAGTCGAGATATTGTTTAGCAGATGGAGTGTTTTCTGTTGAACACTAAGTACTGCCACAAGTTACTTTTTTTTTTTTAAACTTTGAGTATTTTTTTACAATGTTGCTGGAGGTGATCTGTTTATGCTTTGAGAGTGTTCGAATTTAAAATCAGAAAATCATGTCAGTGAGTGAGTCTTTCAAATAATCCTTCGGCATGAAACCTGAGCCTAGTAAACTATGAAAGTAAACTCGGCACATTACCCGAAAGTCTCAATGTCATATTTTCACCCCCATCAATATTATTGATGATTGCTCATTTTCTAATGTGGGACCTGAAATTTACCAGGTGCTTAAAGAATCTTTTTGTTTTTCAGATTCATTGATTCCAGGTAAATCAGAGGAACAAGCAACATGAACAGAAATATGTAGAAAAAGCTATTATGCAGAAGCATAATTGTTGTTTCAGAAGTCCAGCATCTGGTGCACTTAACAATAGAGAATATATTAAACTCTTTCCAAAATAAAAAAAAAAAAAAAAAAAAA |
pcDNA3.1/FBXL19 | ATGGGTATGAAAGTCCCCGGAAAGGGGGAATCTGGGCCCTCGGCGTTGCTGACGCCCCCAATGTCGTCGAGCAGCCGGGGGCCGGGGGCCGGAGCGCGCCGACGCCGAACCCGCTGCCGCCGCTGCCGGGCCTGTGTGCGAACTGAGTGCGGGGATTGCCACTTCTGCCGAGACATGAAGAAGTTCGGGGGGCCCGGGCGCATGAAGCAGTCGTGCCTGCTCCGGCAGTGCACTGCCCCCGTGCTCCCACACACAGCTGTGTGCCTCTTGTGTGGGGAGGCTGGGAAGGAGGACACGGTGGAGGGAGAGGAAGAGAAATTTGGTTTGAGCCTCATGGAGTGTACAATCTGCAACGAGATCGTCCACCCCGGCTGCCTGAAGATGGGGAAGGCTGAGGGTGTCATCAATGCAGAGATCCCCAACTGCTGGGAGTGCCCTCGCTGCACCCAGGAAGGCCGCACCAGCAAGGATTCAGGTGAGGGGCCTGGCCGCCGTAGGGCCGACAACGGCGAGGAGGGCGCCAGCTTGGGGAGCGGATGGAAGCTGACAGAGGAGCCACCGCTTCCACCGCCCCCGCCCAGGCGCAAGGGCCCCCTGCCTGCCGGGCCCCCCCCGGAGGACGTGCCTGGGCCCCCCAAACGAAAGGAAAGGGAGGCAGGGAATGAGCCTCCCACCCCAAGGAAAAAGGTGAAAGGAGGCCGAGAGAGGCACCTGAAGAAGGTGGGTGGAGACGCCTGCCTCCTCCGAGGATCGGACCCAGGCGGCCCGGGCCTGCTGCCCCCCAGGGTTCTGAATCCGAGCCAGGCTTTCTCATCCTGCCACCCTGGGCTCCCTCCCGAGAACTGGGAGAAACCAAAGCCGCCTTTGGCCTCTGCAGAGGGCCCAGCGGTGCCGTCCCCGTCCCCGCAGAGGGAGAAGCTAGAGCGTTTCAAGCGGATGTGCCAGCTGCTGGAACGGGTGCCTGACACCTCCTCTTCCTCCTCGGACTCAGACTCCGACTCCGACTCTTCGGGCACATCGCTGAGTGAGGACGAAGCCCCCGGCGAGGCCCGGAATGGGCGACGGCCAGCCCGGGGCAGCTCTGGCGAGAAGGAGAACCGTGGGGGGCGGCGGGCTGTGCGCCCTGGCAGTGGGGGGCCCCTACTCAGCTGGCCCCTGGGCCCAGCCCCACCACCCCGGCCTCCACAGCTGGAGCGGCACGTGGTGCGGCCCCCGCCTCGAAGCCCTGAGCCCGACACACTCCCCTTGGCTGCTGGATCCGACCACCCCCTGCCCCGGGCCGCCTGGCTTCGCGTCTTCCAGCACCTCGGGCCGCGGGAGCTGTGTATCTGCATGCGAGTCTGCCGAACTTGGAGCCGCTGGTGCTATGACAAGCGTCTGTGGCCTCGAATGGACCTGAGCCGGCGGAAGTCACTGACCCCGCCCATGCTCAGTGGTGTGGTTCGCCGCCAGCCCCGTGCCCTGGACCTCAGCTGGACAGGTGTCTCCAAGAAGCAGCTCATGTGGCTTCTGAACCGACTACAAGGCCTGCAGGAGCTGGTGCTCTCTGGCTGCTCCTGGCTCTCTGTCTCTGCCCTGGGCTCAGCCCCACTGCCAGCCCTGCGGCTCCTGGACCTCCGCTGGATCGAGGATGTTAAAGACTCCCAGCTCCGGGAGTTGCTGCTGCCTCCACCAGACACCAAACCAGGGCAAACAGAGAGCCGTGGTCGGCTGCAGGGGGTGGCAGAACTGCGTCTGGCAGGTTTGGAGCTGACAGATGCCTCCCTGCGTCTCCTGCTGCGTCACGCACCCCAGCTGAGCGCCCTGGACCTGAGCCACTGCGCCCACGTCGGGGACCCCAGTGTTCACCTCCTCACGGCCCCCACGTCCCCACTCCGCGAGACCCTGGTGCACCTCAATCTTGCTGGTTGCCACCGCCTAACGGACCACTGCCTCCCGCTGTTCCGCCGCTGCCCTCGTCTACGCCGCCTAGACCTGCGCTCCTGCCGCCAGCTCTCACCCGAAGCTTGTGCCCGGCTGGCAGCTGCCGGGCCCCCTGGCCCCTTCCGCTGCCCTGAGGAGAAGCTGCTTCTCAAGGACAGCTAG |