Fig. 3
From: MiR-4310 induced by SP1 targets PTEN to promote glioma progression
miR-4310 directly targets PTEN. a Schematic diagram of putative binding sequences of miR-4310 in the 3′-UTR of PTEN. b PTEN mRNA expression was detected by qPCR in miR-4310-overexpressing U87 and LN229 cells. c PTEN protein level was detected in U87 and LN229 cells under LV-miR-4310 transfection or co-transfection with miR-4310 inhibitor. d PTEN expression was evaluated by IHC staining in xenografts derived from U87-LV-NC and U87-LV-miR-4310 cells. e Luciferase reporter assay was used to determine the miR-4310 binding sites in the PTEN 3′-UTR. Data were presented as mean ± s.d. NS no statistical significance, *p<0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001