Fig. 4

Overexpression of PTEN can reverse the biological functions mediated by miR-4310. a, b Proliferative ability of U87 and LN229 cells co-transfected with PTEN plasmid and LV-miR-4310 was examined by EdU assays (a) and MTT assays (b). c, d Migration and invasion of U87 and LN229 cells co-transfected with PTEN plasmid and LV-miR-4310 were examined by Transwell assays (c) and Boyden assays (d), respectively. e, f Expression of ZEB1, N -Cadherin, E-Cadherin, PI3K, AKT, p-PI3K and p-AKT were detected by western blot in U87-LV-miR-4310 and LN229-LV-miR-4310 performed after transfection with pENTER plasmid (the control group) and PTEN plasmid (the treatment group) as indicated. GAPDH were used as a loading control. Data were presented as mean ± s.d. NS no statistical significance, *p<0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001