Fig. 2

Effects of SNHG12 knockdown on radiosensitivity in CC cells. SiHa and Hela cells were transfected with si-SNHG12 or si-con. a, b Clonogenic assay was performed to assess the survival fractions of SiHa and Hela cells. c, d The apoptosis of SiHa and Hela cells treated with different doses of radiation (0 Gy and 2 Gy) was determined by flow cytometry. e, f The caspase-3 activity of SiHa and Hela cells treated with different doses of radiation (0 Gy and 2 Gy) was detected by Caspase-3 Activity Assay Kit. g, h Flow cytometry was used to examine the cell cycle distribution in SiHa and Hela cells treated with different doses of radiation (0 Gy and 2 Gy). i, j WB analysis was performed to measure the protein expression of CCND1 in SiHa and Hela cells treated with different doses of radiation (0 Gy and 2 Gy). *P < 0.05