Fig. 6

Effects of miR-148a inhibitor and CDK1 overexpression on radiosensitivity, apoptosis and cell cycle in CC cells. SiHa and Hela cells were transfected with si-SNHG12 and anti-miR-148a or CDK1 overexpression plasmid. a, b The survival fractions of SiHa and Hela cells were assessed by clonogenic assay. c, d Flow cytometry was used to determine the apoptosis of SiHa and Hela cells treated with 2 Gy radiation. e, f Caspase-3 Activity Assay Kit was used to detect the caspase-3 activity of SiHa and Hela cells treated with 2 Gy radiation. g, h The cell cycle distribution in SiHa and Hela cells treated with 2 Gy radiation was determined using flow cytometry. i, j The protein expression of CCND1 in SiHa and Hela cells treated with 2 Gy radiation was tested by WB analysis. *P < 0.05