Fig. 3

NEAT1 was interacted with miR-27b-3p. a The predicted binding sites or mutant binding sites of NEAT1 in miR-27b-3p. b, c Luciferase reporter assay was carried out to confirm the relationship between miR-27b-3p and NEAT1. d qRT-PCR was performed to examine the expression of miR-27b-3p in GC tissues and normal tissues. b qRT-PCR was performed to examine the expression of miR-27b-3p after the GC patients received radiation treatment. f The expressions of miR-27b-3p in human gastric epithelium cell GES-1 and gastric cancer cell lines (NCI-N87, SGC-7901, MKN-45, and AGS). g, h The expression of miR-27b-3p in AGS and MKN-45 cells treated with 4 Gy radiation for 0 to 24 h. i, j The expression of miR-27b-3p in cells transfected with scrambled, shNEAT1, pcDNA vector, or NEAT1 overexpression plasmid. k The expression correlation between NEAT1 and miR-27b-3p in GC tissues. *P < 0.05