Fig. 4

Wnt response is partly location specific. a Genes upregulated after Wnt activation (fold change > 1.2, adjusted p-value < 0.05, Wald-Test) were identified in each region separately. Genes which appeared significantly upregulated in all locations individually are shown on top, followed by remaining 20 most significantly upregulated genes for each location (intersecting genes shown in between). Heatmap shows average (n = 3) scaled counts per condition, centred around the location average, right columns show summarized log2FC(Cβ/wt) and mean read count across locations. Genes marked with asterix are differentially expressed in the average of all locations (adjusted p-value < 0.05, Wald-Test). b Gene Set Enrichment Analysis of genes upregulated after Wnt activation (fold change > 2, 200 lowest adjusted p-values < 0.05, Wald-Test) in each location using GSEA preranked mode, after genes were ranked based on expression in each location’s expression in wt vs. other locations expression in wt. c The genes upregulated after Wnt activation from each location were examined for overlap with transcription factor target genes using ChEA3, the resulting score was tested for correlation with the expression of the corresponding transcription factor coding gene in each location