Fig. 6

NORAD promotes cell proliferation, invasion and EMT, suppresses cell apoptosis via miR-30a-5p/RAB11A/WNT/β-catenin pathway in PC-3 cells. PC-3 cells were transfected with 50 nM RAB11A siRNA for 48 h. a, b Interference efficiencies of RAB11A siRNA were determined by qRT-PCR and Western blotting, respectively. Subsequently, PC-3 cells were transfected with 2 μg/mL pcDNA-NORAD alone, or together with 50 nM RAB11A siRNA. c After 48 h transfection, CCK-8 was used to detect the cell proliferation. d, e Cell invasion and apoptosis was determined by Transwell and Flow cytometry in PC-3 cells after 48 h transfection with NORAD alone or together with RAB11A siRNA. f, g Western blotting was used to determine the expression levels of related proteins of EMT (N-cadherin, E-cadherin, Vimentin and Snail) and WNT pathway (β-catenin, Cyclin D and c-Myc) in PC-3 cells after 48 h transfection. The data were presented as the mean ± standard error of mean (SEM), n = 3. Student’s t-test was used for the comparison between 2 groups in this study. *P < 0.05