Fig. 2

REV7 is upregulated in 5-FU and oxaliplatin resistant CRC cells. a Western blot analysis of REV7 protein expression in HT29 WT, HT29 5-Fu-R, b SW480 WT, SW480 5-Fu-R, c HT29 Oxa-R and d SW480 Oxa-R cells. Expression of β-actin (Actin) was used as internal control. e Western blot analysis of REV7 protein expression in response to 5-Fu in HT29 WT and f SW480 WT cells. Cells were treated with 0 µM, 0.5 µM, 1 µM and 2 µM of 5-Fu for 48 h before harvesting for western blotting analysis. g Western blot analysis of REV7 protein expression in response to oxaliplatin in HT29 WT and h SW480 WT cells. Cells were treated with 0 µM, 2 µM, 4 µM and 8 µM of oxaliplatin for 48 h before harvesting for western blotting analysis