Fig. 2
From: MAFG-AS1 aggravates the progression of pancreatic cancer by sponging miR-3196 to boost NFIX
MiR-3196 bound with MAFG-AS1. a, b FISH (scale bar = 20 μm) and nucleus cytoplasm fractionation assays located MAFG-AS1 in cytoplasm. c Venn diagram showed 4 mutual miRNAs interacting with MAFG-AS1 that were found after searching starBase, ReGRNA and miRDB databases. d RNA pull down assays detected the interaction of MAFG-AS1 with the selected 4 miRNAs. e MiR-3196 expression was measured in PC cell lines via RT-qPCR. f RIP assays exhibited the interaction between MAFG-AS1 and miR-3196. g Binding sites between miR-3196 and MAFG-AS1 were shown. h RNA pull down examined the binding sites responsible for the interaction between miR-3196 and MAFG-AS1. i RT-qPCR detectedmiR-3196 expression in PC cells transfected with miR-3196 mimics or NC mimics. j Luciferase reporter assays appraised the binding between miR-3196 and MAFG-AS1. **P < 0.01