Fig. 4
From: MAFG-AS1 aggravates the progression of pancreatic cancer by sponging miR-3196 to boost NFIX
MiR-3196 bound with NFIX 3′ UTR. a The expression of three mRNAs was assessed by RT-qPCR in cells with or without MAFG-AS1 inhibition. b NFIX expression was detected by RT-qPCR in cells with miR-3196 mimics or NC mimics. c NFIX expression was measured by RT-qPCR in cells transfected with sh-NC, sh-MAFG-AS1#1 or sh-MAFG-AS1#1 plus miR-3196 inhibitor. d NFIX expression was detected in PC cell lines through RT-qPCR. e RIP assay results showed that MAFG-AS1, miR-3196 and NFIX were enriched by Ago2 antibody. f RNA pull down examined miR-3196 bound with NFIX. g Luciferase reporter assays validated the binding between miR-3196 and NFIX. *P < 0.05, **P < 0.01