Fig. 4

Aurora-B knockdown-induced autophagy inhibits migration and invasion in OS cells. The migration and invasion ability of Aurora-B-knockdown 143B and HOS cells treated with or without CQ (12 μM, 12 h) were examined by transwell migration (a, b ***P < 0.01, Scale bars: 100 μm) and invasion (c, d *P < 0.05, ****P < 0.01, ***P < 0.01,**P < 0.01, Scale bars: 100 μm) assays in vitro. Wound healing assay was performed with scrambled and shAurora-B 143B and HOS cells treated with CQ (12 μM, 12 h) for 24 h or not (e, f **P < 0.01, ***P < 0.01, *P < 0.05, Scale bars: 100 μm). MMP2 protein expression of Aurora-B-knockdown cells treated with CQ (10 μM, 24 h) or not was analyzed by western blotting (g)