Fig. 2

LINC01194 is capable to combine with miR-641. a, b The nuclear/cytoplasmic separation assay and FISH assay was used to confirm the subcellular localization of LINC01194 in LUAD cells. c RIP was applied to detect the enrichment of LINC01194 by anti-Ago2 or anti-IgG. d The screening condition of LINC01194-interacting miRNAs by starBase v2.0. e qRT-PCR analysis was conducted to detect the overexpression efficiency of miR-461. f The potential binding sites between LINC01194 and miR-641 were hypothesized by starBase v2.0. g RNA pull down assay confirmed the binding of miR-641 to the site 2 of LINC01194. h qRT-PCR analysis was conducted to detect the overexpression efficiency of miR-641 in two LUAD cells. i Luciferase reporter assays were used to confirm the interaction between LINC01194 and miR-641. All results were displayed as the mean ± SD, n = 3, Student’s t test or one-way analysis of variance was carried out for evaluating the differences between groups. *P < 0.05, **P < 0.01