Fig. 4

LINC01194 serves as a ceRNA to elevate the expression of SETD7 by competing for miR-641. a StarBase v2.0 screened out 6 targets of miR-641 that shared by PITA, miRmap and RNA22 tools. b The influences of miR-641 on above selected 6 gens were detected by qRT-PCR. c The qRT-PCR was used to analyze the expression of SETD7 after transfection with sh-LINC01194#1&2. d The expression of SETD7 in LUAD cell lines relative to the normal 16HBE cells was tested by qRT-PCR. e, f RIP and pull down assays to detect three RNAs whether co-exist in RISC and combined each other. g StarBase v2.0 predicted the possible binding sites between miR-641 and SETD7. h qRT-PCR was used to access the interference efficiency of miR-641. i Luciferase reporter assay was used to confirm the interaction between miR-641 and SETD7. j SETD7 expression in indicated LUAD cells was examined via qRT-PCR. All results were displayed as the mean ± SD, n = 3, comparisons between groups were subjected to Student’s t test or one-way ANOVA. **P < 0.01