Fig. 3
From: SKP1 promotes YAP-mediated colorectal cancer stemness via suppressing RASSF1
Knockdown of SKP1 inhibits the stemness and EMT of colorectal cancer cells. a–c Knockdown of SKP1 inhibits the stemness of colorectal cancer cells. The sphere-forming capacity (a), frequency of sphere-forming cells (b), and expression of cancer stem cell markers (c) of SKP1-knockdown and control HCT-116 and #1 primary colorectal cancer cells were determined by sphere formation (a), limiting dilution assay (b), and qRT-PCR (c), respectively. Knockdown of SKP1 inhibits EMT of colorectal cancer cells. The migration (d) and expression of EMT markers (e) of SKP1-knockdown and control HCT-116 and #1 primary colorectal cancer cells were determined by tranwell (d) and qRT-PCR assay (e), respectively. Student’s t-test (***p < 0.001)