Fig. 4
From: SKP1 promotes YAP-mediated colorectal cancer stemness via suppressing RASSF1
SKP1 positively regulates Hippo/YAP signaling pathway in colorectal cancer cells. a The expression of SKP1 is positively correlated with YAP target genes as analyzed by spearman analysis with TCGA datasets. The TCGA dataset was downloaded from R2 platform. Spearman analysis was employed for investigation of the correlation between SKP1 and YAP target genes. b The expression of SKP1 positively correlates with YAP target genes as analyzed by spearman analysis with RNA isolated from 30 cancer tissues of colorectal cancer patients. The total RNA was extracted from clinical samples. The mRNA levels of indicated genes were analyzed by qRT-PCR. Spearman analysis was employed for investigation of the correlation between SKP1 and YAP target genes. c The mRNA levels of YAP target genes were upregulated by SKP1 overexpression in colorectal cancer cells. The mRNA of YAP target genes in indicated cells were analyzed by qRT-PCR. d The phosphorylation levels of YAP in indicated cells were analyzed by western blot. e The protein level of nuclear YAP in indicated cells was analyzed by western blot. f SKP1 promotes nuclear translocation of YAP in colorectal cancer cells. The localization of YAP in indicated cells was analyzed by immunostaining. Student’s t-test (***p < 0.001)