Fig. 6
From: SKP1 promotes YAP-mediated colorectal cancer stemness via suppressing RASSF1
SKP1 negatively regulates RASSF1 at both mRNA and protein level. a The correlation between SKP1 and RASSF1 was analyzed by TCGA dataset (left) and clinical samples (right). b The mRNA levels of RASSF1 in indicated cells were analyzed by qRT-PCR. c Characterization of SKP1-overexpressing HT-29 and #2 primary cells by western blot. d The mRNA levels of RASSF1 in indicated cells were analzyed by qRT-PCR. e, f The transcriptional activity of the promoter of RASSF1 (e) and different fraction of RASSF1 promoter (f) in indicated cells were analyzed by luciferase reporter assay. g The transcription of RASSF1 in indicated cells was analyzed by nuclear run-on assay. h The nuclear SKP1 in indicated cells was analyzed by western blot assay. i The binding between SKP1 and RASSF1 promoter was analyzed by ChIP-PCR assay. j, k The RASSF1 protein levels in indicated cells were analyzed by western blot assay. l, m The binding between SKP1 and RASSF1 (l), RASSF1 and Cullin-1 (m) were analyzed by immunoprecipitation assay. Student’s t-test (***p < 0.001)