Fig. 7
From: SKP1 promotes YAP-mediated colorectal cancer stemness via suppressing RASSF1
Overexpression of RASSF1 abolished the effect of SKP1 on Hippo/YAP signaling, stemness and EMT of colorectal cancer cells. a Characterization of SKP1-overexpressing RASSF-overexpressing colorectal cancer cells. b The mRNA levels of YAP target genes in indicated cells were analyzed by qRT-PCR. c–f The sphere-forming capacities (c), frequency of sphere-forming cells (d) and migration capacity (e) in indicated cells were analyzed by sphere formation (c), limiting dilution (d) and transwell (e) assay, respectively. f The mRNA levels of cancer stem cell markers (left) and EMT markers (right) in indicated cells were analzyed by qRT-PCR. Student’s t-test (***p < 0.001)