Fig. 3

CBR3-AS1 facilitates Wnt/β-catenin signaling activation by promoting nuclear location of β-catenin in LAD cell lines. a A549 cells were transfected with control siRNA or CBR3-AS1 siRNA and the expression of β-catenin was examined by western blotting with antibody against β-catenin. b A549 cells were transfected with control siRNA or CBR3-AS1 siRNA and the expression of CBR3-AS1 and β-catenin was examined by qRT-PCR assays. Each bar represents the mean ± SD for biological triplicate experiments. **P < 0.01, one-way ANOVA. c A549 cells were transfected with control siRNA or CBR3-AS1 siRNA and the cellular components were extracted followed by western blotting with antibody against β-catenin. d TopFlash/FopFlash-luciferase reporter constructs are as shown (upper panel). A549cells were co-transfected with control siRNA or CBR3-AS1siRNA together with renilla and pGL3-TopFlash orpGL3-FopFlash. These cells were cultured in the absence or presence of 25 mM LiCl for 4 h before measuring luciferase activity. The relative luciferase activity was normalized with values of renilla luciferase and pGL3-FopFlash luciferase activity. Western blotting against with indicated antibodies. Each bar represents the mean ± SD for biological triplicate experiments. **P < 0.01, one-way ANOVA. e A549 cells were transfected with control siRNA or CBR3-AS1 siRNA and the expression of CBR3-AS1, c-Myc, LGR5 and MMP-7 was examined by qRT-PCR assays. Each bar represents the mean ± SD for biological triplicate experiments. **P < 0.01, one-way ANOVA