Fig. 2

ELK4 stimulated transcription of HCG11 in VS cells. a The motif structure was projected by bioinformatics. b RT-qPCR was built to determine the expression of ELK4 in cells treated with pcDNA3.1/ELK4. c HCG11 expression was evaluated in cells treated with pcDNA3.1/ELK4. d Luciferase reporter assays were carried out to verify the combination between ELK4 and HCG11. e ChIP assays were established to certify the affinity between ELK4 and HCG11. f Luciferase reporter assays were performed to testify the relationship between ELK4 and HCG11 PMT. g ChIP assays were used to confirm the interaction between ELK4 and HCG11 PMT. h Luciferase reporter assays were constructed to find out the binding place between ELK4 and HCG11 PMT. *P < 0.05, **P < 0.01