Fig. 3

The effects of GPX8 on the Wnt signaling pathway. a, b The expression levels of key proteins (wnt1, wnt3a, p-GSK3β, GSK3β and β-catenin) of the Wnt signaling pathway were examined using western blot in normal human gastric epithelial cells (GES-1) and Gastric cancer cell lines (NCI-N87, SNU-16, SNU-5 and HGC-27). c, d After HGC-27 cells were transfected with blank control vector, pcDNA-control, pcDNA-GPX8, si-NC and si-GPX8, the expression levels of wnt1, wnt3a, p-GSK3β, GSK3β and β-catenin were detected using western blot. After pcDNA-GPX8 combined with or without Wnt inhibitor was co-transfected into HGC-27 cells, c the cellular proliferation was assessed by CCK-8 assay; f Colony formation assay, transwell migration and invasion assays were utilized to respectively detect the cellular colony formation, migration and invasion abilities. The images of data quantification of g colony formation, h transwell migration and i migration assays. ****p < 0.0001 Versus GES-1 or pcDNA-control; ^###p < 0.001, ^####p < 0.0001 versus si-NC or pcDNA-GPX8