Fig. 5

The roles of FOXC1 in gastric cancer cells. The a, b protein and c mRNA expression levels of FOXC1 in normal human gastric epithelial cells (GES-1) and gastric cancer cell lines (NCI-N87, SNU-16, SNU-5 and HGC-27) were determined using western blot and qRT-PCR. HGC-27 cells were transfected with blank control vector, pcDNA-control, pcDNA-FOXC1, si-NC and si-FOXC1, respectively, and then the d, e protein and f mRNA expression levels of FOXC1 were assessed by western blot and qRT-PCR. g The cellular proliferation was examined by CCK-8 assay; h The colony formation, migration and migration abilities were respectively measured using colony formation assay, transwell migration and invasion assays. The images of data quantification of i colony formation, j transwell migration and (K) migration assays. ***p < 0.001, ****p < 0.0001 Versus GES-1 or pcDNA-control; ^##p < 0.01, ^###p < 0.001, ^####p < 0.0001 versus si-NC