Fig. 6

The relationship between FOXC1 and GPX8. The binding relationship of GPX8 with FOXC1 was determined by a dual luciferase reporter and b, c chromatin immunoprecipitation (CHIP) assays. After HGC-27 cells were transfected with pcDNA-control, pcDNA-FOXC1, si-NC or si-FOXC1, the d, e protein and f mRNA expression levels of GPX8 were detected by western blot and qRT-PCR. After HGC-27 cells were respectively transfected with pcDNA-control + si-NC, pcDNA-GPX8 + si-NC and pcDNA-GPX8 + si-FOXC1, g CCK-8 was used to determine the cell viability; h colony formation assay, transwell migration and invasion assays were applied to detect the cell proliferation, migration and invasion. The images of data quantification of i colony formation, (J) migration and (K) migration assays. *p < 0.05, **p < 0.01, ***p < 0.001, ^****p < 0.0001 Versus pcDNA-control, lgG or pcDNA-control + si-NC; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, ^####p < 0.0001 Versus si-NC or pcDNA-GPX8 + si-NC