Fig. 7

The effects of GPX8 on tumor growth. HGC-27 cells were respectively transfected with blank control vector, pcDNA-control, pcDNA-GPX8, sh-NC and sh-GPX8, collected and subcutaneously injected into mice. After inoculation, the tumor sizes were recorded every seven days, and the mice were euthanized and sacrificed on day 28. a The expression of GPX8 and Ki-67 in the mice tumors were determined by immunohistochemistry (IHC) assay. b, c The images of positive expression quantification of GPX8 and Ki-67 in IHC assay. d The images of the mice tumors on day 28. e The changes of tumor volumes during 28 days of feeding. *p < 0.05, **p < 0.01, ****p < 0.0001 Versus pcDNA-control; ^##p < 0.01, ^####p < 0.0001 versus si-NC