Fig. 2From: Circular RNA circPLK1 promotes breast cancer cell proliferation, migration and invasion by regulating miR-4500/IGF1 axis CircPLK1 knockdown limited BC cell growth, migration and invasion. BT549 and HCC38 cells were transfected with sh-NC or sh-circPLK1. a Knockdown efficiency of circPLK1 was evaluated by qRT-PCR. b CCK-8 assay was employed to evaluate cell viability. c, d Flow cytometry was applied to analyze the cell cycle distribution. e Western blot assay was conducted to examine the protein expression of CDK4 and CDK6. f, g Transwell assay was utilized to count the number of migrated and invaded cells (100 ×). ***P < 0.001Back to article page