Fig. 5

Interaction between miR-4500 and circPLK1 or IGF1 in BC cells. a StarBase v3.0 predicted the complementary sequences between circPLK1 and miR-4500. b BT549 and HCC38 cells were co-transfected with wt-circPLK1 or mut-circPLK1 and miR-NC or miR-4500, and then relative luciferase activity was measured. c Relative expression of circPLK1 was examined by RNA pull-down assay in BT549 and HCC38 cells incubated with Bio-miR-NC or Bio-miR-4500. d, e The expression of miR-4500 was examined by qRT-PCR and western blot analyses in BC tissues, adjacent normal tissues, BC cells, and MCF-10A cells. f Overexpression efficiency of circPLK1 was determined by qRT-PCR in BT549 and HCC38 cells transfected with Vector or circPLK1. g The level of miR-4500 was detected by qRT-PCR in BT549 and HCC38 cells transfected with sh-NC, sh-circPLK1, Vector, or circPLK1. h The putative binding sites between miR-4500 and IGF1 were predicted by starBase v3.0. i The luciferase activity in BT549 and HCC38 cells co-transfected wt-IGF1 or mut-IGF1 and miR-NC or miR-4500 was examined. j The mRNA expression of IGF1 was determined by RNA pull-down assay in BT549 and HCC38 cells incubated with Bio-miR-NC or Bio-miR-4500. k Knockdown efficiency of miR-4500 was determined by qRT-PCR in BT549 and HCC38 cells transfected with anti-NC or anti-miR-4500. ***P < 0.001