Fig. 2

Knockdown of YTHDF1 inhibits proliferation and sensitizes GBM cells to TMZ. a qRT-PCR showing the expression levels of YTHDF1 mRNA in DBTRG-05MG cells transfected with two siRNAs (siYTHDF1#1 and siYTHDF1#2). Data are expressed relative to cells transfected with control siRNA (siCtl). Means from three separate experiments are shown with SD error bars. **P < 0.01 vs. siCtl (Student’s t-test). b Western blotting showing protein expression levels of YTHDF1 in DBTRG-05MG cells with YTHDF1 knockdown by two siRNAs (siYTHDF1#1 and siYTHDF1#2) and control (siCtl). YTHDF2 and YTHDF2 are shown as a control for siRNA specificity, GAPDH was used as a loading control. c Cell proliferation determined by MTT assay performed on control (siCtl) and YTHDF1-silenced (siYTHDF#1 and siYTHDF1#2) DBTRG-05MG cells. The proliferation rate was calculated as the mean ratio of MTT absorbance on days 2 and 3 to that on day 1. Means from three separate experiments are shown with SD error bars. **P < 0.01 comparing siYTHDF1 vs. siCtl (Student’s t-test). d Cell viability determined by MTT assay performed on control (siCtl) and YTHDF1-silenced (siYTHDF#1 and siYTHDF1#2) DBTRG-05MG cells treated with the indicated concentrations of TMZ for 16 h. Data expressed as mean ratios of MTT absorbances at 0.5 mM, 2 mM, and 3 mM TMZ to that at 0 mM TMZ. Three independent experiments were performed and SD error bars are shown, **P < 0.01 (Student’s t-test)