Fig. 5

YTHDF1 mediates MSI1-dependent enhancement of cell proliferation. a Cell proliferation assay performed on DBTRG-05MG cells transfected with scrambled control (siScr) and MSI1-targeting siRNA (siMSI1). b Cell proliferation assay performed on DBTRG-05MG cells transfected with control siRNA (siCtl) and two YTHDF1-targeting siRNAs (siYTHDF1#1 and siYTHDF1#2). c Cell proliferation assay performed on DBTRG-05MG cells transfected with the indicated combinations of MSI1-overexpressing plasmid (MSI1) and YTHDF1-targeting siRNAs (siYTHDF1#1 and siYTHDF1#2). siScr – siRNA control, vec – overexpression plasmid control. The proliferation rate in a, b, and c was calculated as the mean ratio of MTT absorbance on the indicated days to that on day 1. Means from three separate experiments are shown with SD error bars. d qRT-PCR validation of overexpression of MSI1 (left panel) and knockdown of YTHDF1 (right panel) in the experiment shown in c. All data are rpesented as means from three separate experiments with SD error bars. *P < 0.05, **P < 0.01, ***P < 0.005 (Student’s t-test)