Fig. 6

YTHDF1 mediates MSI1-dependent enhancement of cell mobility. a Wound healing assay performed on DBTRG-05MG cells transfected with siRNA scrambled control (siScr) and MSI1-targeting siRNA (siMSI1). b Wound healing assay performed on DBTRG-05MG cells transfected with siRNA scrambled control (siScr) and MSI1-targeting siRNA (siMSI1). In a and b, left panel: images of wounds at the beginning (0 hr) and end (24 hr) of the assay; middle panel: quantification of migratory cells in a time course of 24 h; right panel: quantification of migratory cells at the end point of the assay (24 h). c Transwell migration assay performed on DBTRG-05MG cells transfected with empty vector control (vec) and MSI1-encoding plasmid (MSI1). Top panel: fluorescent images of migrated cells stained with propidium iodide (triplicate). Bottom panel: quantification of migrated cells. d Wound healing (top two panels) and transwell migration (bottom panel) assays performed on DBTRG-05MG cells transfected with the indicated combinations of siRNAs (siScr and siYTHDF1) and plasmids (vec and MSI1). Right panel: quantification of the assays. All quantifiable data are presented as the means from three biological replicates with SD error bars. *P < 0.05, **P < 0.01, ***P < 0.005 (Student’s t-test)